Standard curves for BSA standards in the BCA Protein Assay measured at selected wavelengths above and below the optimum (562 nm). Compared to most dye-binding methods, the BCA Assay is affected much less by protein compositional differences, providing greater protein-to-protein uniformity. The BCA Protein Assay combines the well-known reduction of Cu 2+ to Cu 1+ by protein in an alkaline medium with the highly sensitive and selective colorimetric detection of the cuprous cation (Cu 1+) by bicinchoninic acid (BCA). Thermo Scientific Pierce 660 nm Protein Assay 13 Overview 13 Highlights 13 Typical Response Curves 14 . Add to Wish List. PREPARATION NOTE: Store kit contents at room temperature. 23227. PI23227 Print Thermo Scientific Pierce BCA Protein Assay Kit Green-to-blue (A562nm), precise, detergent-compatible assay reagent to measure total protein concentration vs. protein standard. of Assays/Kit: 250 Tube Assays/2500 Microplate Assays Yield . Current versions of product instructions are available at . Rating: 0 out of 100 - based on 0 review (s) | Write Review |. Stock: Pre order. Catalog no: 23227; Pierce BCA Protein Assay Kit; Full product specification; Estimated delivery period upon order confirmation (complete payment): -Ex-stock: 7-10 days-Out of stock: 11-30 days The first step is the chelation of copper with protein in an alkaline environment to form a light blue complex. PierceTM BCA protein assay kit Pierce TM BCA . Learn more about 23227. Blue and pink edges are known interactions (from curated databases and experimentally determined, respectively). BCA Protein Assay kit (Pierce catalog #'s 23225 and 23227) 3. : 23227. 23235 Pierce Micro BCA Protein Assay Kit, working range of 0.5-20 g/ml . The Micro BCA Protein Assay Standard Protocol: 60C for 60 minutes 0.5-20 g/ml (0.5 ml) (A) on tau protein levels of A152T and control neurons. 23225 BCA Protein Assay Reagent Kit, sufficient reagents for 500 test tube or 5,000 microplate assays 23227 BCA Protein Assay Reagent Kit, sufficient reagents for 250 test tube or 2,500 microplate assays Kit Contents: 23228 BCA Reagent A, 500 ml . Manufacturer: Thermo Scientific 0023228 View more versions of this product Catalog No. The Pierce BCA Protein Assay can be used to assess yields in whole cell lysates and affinity-column fractions, as well as to monitor protein contamination in industrial applications. Bel-Art disposable pestles Cat. The Thermo Scientific Pierce BCA Protein Assay Kit is a two-component, high-precision, detergent-compatible assay reagent set to measure (A562nm) total protein. 23227), containing sodium carbonate, sodium bicarbonate, bicinchoninic acid and sodium tartrate in 0.1 M sodium hydroxide No. Fort Pierce, FL 34949. . The levels of chemokine (C-X-C motif) ligand 1 (CXCL1 . PIERCE BCA PROTEIN ASSAY KIT RE232690 CATALOG#23227 (BCA KIT) United States: Banglore Air Cargo: NOS: 2: 18,458: 9,229: Sep 23 2016: 38220090: QPBCA-1KT QUANTIPRO BCA ASSAY KIT [FOR LAB PURPOSE] United States: Banglore Air Cargo: NOS: 1: 22,491: 22,491: Sep 22 2016: 38220090: BCA PROTIEN ASSAY KIT ( LAB REAGENT FOR RESEARCH USE ONLY ) United . Sort by Relevance. 23236) Every protein assay has limitations depending on the application and the specific protein sample analyzed. Telephone: 800-8-PIERCE (800-874-3723) or 815-968-0747 Fax: 815-968-7316 or 800-842-5007 . Overview of Western Blotting. All of the lysis buffers and reagents were tested at various dilutions, ranging from 1X to 0.001X. Equal amounts of protein samples were loaded on SDS-PAGE gels and then transferred to PVDF (polyvinylidene fluoride) membranes. It may be surprising to learn that the history of the western blot only stretches back to the late 1970s. The levels of IL-17A and IL-1 were determined using a multi-factor kit LEGENDplex Mouse Inflammation Panel (13-plex) with a filter plate (740150; Biolegend, Fell, Germany). View MAN0011430_Pierce_BCA_Protein_Asy_UG.pdf from NMMJ BNKJV at St. John's University. 23225 23227 . Pierce Biotechnology PO Box 117 (815) 968-0747 www.thermo.com/pierce 3747 N. Meridian Road Rockford, lL 61105 USA (815) 968-7316 fax 2 Coomassie Plus (Bradford) Protein Assay (Product No. . Manufacturer: Thermo Scientific 0023227 View more versions of this product Catalog No. 23227; Thermo Fisher Scientic Inc., Waltham, Table 1. b. The brain obtained from the APP/PS1 mice were homogenized in ice-cold PBS, and the protein concentration was detected using the Pierce bicinchoninic acid (BCA) protein assay kit (23227; Thermo Fisher Scientific, USA). 23225) or 500 ml (in Product No. An adaptation of Pierce's BCA Protein Assay Reagent Kit (Product No. Alice L. Herneisen and Sebastian Lourido. CERTIFICATE OF ANALYSIS PRODUCT NAME: Pierce BCA Protein Assay Kit CERTIFICATE OF ANALYSIS Products are warranted to operate or perform substantially in conformance with published Product specifications in effect at the time of sale, as set forth in the Product documentation, specifications and/or accompanying package inserts ("Documentation"). Features and Benefits Accurate across a broad range of protein concentrations Manufacturer: Thermo Scientific 23227 View more versions of this product Catalog No. 23227 BCA Protein Assay Kit, sufficient reagents for 250 test tube or 2,500 microplate assays Kit Contents: BCA Reagent A, 1,000 ml (in Product No. Light green and black edges are interactions derived from text mining and co-expression, respectively. Similar Protocol. Description SDS Pricing; 71285-M: The BCA protein assay is a simple and reliable protein quantification method. Bio-protocol() . USER GUIDE Pierce BCA Protein Assay Kit Catalog Numbers 23225 and 23227 Doc. protein Cu (Cu 2+, Cu 1+) reduction , protein reduction Cu BCA , (562nm) Cu BCA . Pierce BCA Protein Assay Kits BCA Protein Assay Kit. PI23227 $251.39 / Each Quantity Add to cart Description Specifications The kit can be used to measure very dilute protein concentrations in very small sample volumes. Analysis of total tau (TAU5) and phospho-tau (S396 P-tau) levels upon treatment by western blot (B) and ELISA (C).Analysis of total tau (TAU5) and phospho-tau (S396 P-tau) levels upon treatment with the negative control QC-03-075 (D), by western blot (E) and ELISA (F).Representative western blots are shown (B, E) with mean densitometry . 9 $192.02 B6916-500ML. Other Pierce products are available in stock. 500 L of PBS to remove dead cells. #LC6070). Thermo Pierce BCA23225 23227Thermo Scientific BCA . MAN0011430 Rev. Published online: 2021-11-05. Casino Fort Pierce offers daily games and races from around the world. The Bicinchoninic Acid (BCA) protein assay is a highly sensitive colorimetric assay that is compatible with . Supernatant was collected and the protein concentration was determined using a Pierce BCA protein assay kit (cat. 23235 Micro BCA Assay Reagent Kit . Keyword:'pierce bca protein assay kit' Showing 1-19 of 19 results for " pierce bca protein assay kit " within Products. 23225) or 500 ml (in Product No. The Pierce BCA Protein Assay is a detergent-compatible formulation based on bicinchoninic acid (BCA) for the colorimetric detection and quantitation of total protein. Compared to most dye-binding methods, the BCA Assay is affected much less by protein compositional differences, providing greater protein-to-protein uniformity. Related Documents Document Title Document Number 96-well DNA Quantification using the dsDNA Quant-iT High Sensitivity Assy Kit and VICTOR3V LIBPR.0108 VII.Safety All Laboratory Safety procedures will be complied with during this procedure. 23227Pierce BCA Protein Assay Kit500ml . Part No. Pierce BCA Protein Assay Kit (23,227, Thermo Fisher Scientific, Waltham, MA), NP40 lysis buffer (FNN0021, Invitrogen, Waltham, MA), protease inhibitor cocktail (P8340, Millipore Sigma, St. Louis, MO), phosphatase inhibitor cocktail (P0044, Millipore Sigma, St. Louis, MO). (Optional) Determine the peptide concentration and yield with a peptide quantitation assay. The Pierce BCA Protein Assay is one of the most commonly used protein quantitation assays, so we have determined the compatibility of this assay with each of the lysis buffers or the reagents containing a lytic agent used in Promega reporter assays. BCA Protein Assay (Pierce Cat.# 23225, 23227) PREPARATION Note: Store kit contents at room temperature. Related products Product Cat. $173.40 - $268.50 Products 2 Description Description Thermo Scientific Pierce BCA Protein Assay Kit remains one of the most popular protein quantitation methods worldwide. Total protein was quantified by Pierce BCA Protein assay (Thermo 491 Fisher Scientific, Waltham, MA, USA), denaturated and equal amounts were loaded in 492 SDS-PAGE. . Compare Product No. The absorbance at 570 nm was quantified in a plate reader (IWAKI EZS-ABS . Pierce* BCA Protein Assay Kit (Thermo Scientific) View Detail Add to Order. At 44 h post transfection, remove the growth media from each well of cultured cells and gently rinse each well with approx. RM 627.00. protocol Protein assay PIERCE protein assay kit BCA Protein assay kit (Cat no. 1 kit / 34,000 750 ml / 25,000 660 nm Protein Assay Reagent, Pierce PCC 22660 500 5,000 . For example, a twofold dilution series from 2000 g/mL to Email me suggestions! #A955). 23227), the Micro BCA Kit has been optimized for use with dilute protein samples (0.5-20 g/ml . BCA Protein Assay Kit 23227 Pierce Quantitative Fluorometric Peptide Assay 23290 Pierce Quantitative Colorimetric Peptide Assay 23275 TMTsixplex Isobaric Label Reagent Set 90061 TMTsixplex Isobaric Mass . The calibration curve was prepared with the . They are not intended for medicinal, . 5. Size: 500 mL No. Used in more labs than any other detergent-compatible protein assay, Pierce BCA Reagents provide accurate determination of protein . The practical range of the quantification is from approx. PierceA Protein Assay Kit BC Catalog Numbers 23225 and 23227 Doc. SP3 peptide cleanup. 23225, 23227 Pierce BCA Protein Assay Kits, working range 20-2000 g/ml . pierce rapid gold bca protein assay kit 250 ml a53226 500 ml a53225 pierce bca protein assay kit 500 ml 23227 1 l 23225 pierce microplate bca protein assay kit - reducing agent compatible 275 ml 23252 pierce micro bca protein assay kit 500 ml 23235 pierce modified lowry protein assay kit 530 ml 23240 pierce detergent compatible bradford assay kit For the assay, protein standards are made in ultrapure water as the diluent. #23227) protein assay . This method combines the well-known reduction of Cu+2 to Cu+1 by protein in an alkaline medium (the biuret reaction) with the highly sensitive and selective colorimetric detection . Acetonitrile (MeCN) (Fisher Chemical Optima/LC-MS grade Cat. 23227 BCA Protein Assay Kit, sufficient reagents for 250 test tube or 2,500 microplate assays Kit Contents . For telomerase activity, HBMECs were lysed as per the protocol, and . 78410 Halt Protease Inhibitor Cocktail Kit 78248 B-PER Bacterial Protein Extraction Reagent, 500 ml 78990 Y-PER Yeast Protein Extraction Reagent, 500 ml 89826 Mem-PER Membrane Protein Extraction Reagent Kit 23236 Coomassie Plus The Better Bradford Assay 23227 Pierce BCA Protein Assay Kit 78833 NE-PER Nuclear and Cytoplasmic Extraction Kit This is too concentrated to be assayed by the Coomassie Plus Protein Assay Kit, whose assay range in the standard microplate protocol is 100-1500g/mL. 23227), containing sodium carbonate, sodium bicarbonate, bicinchoninic acid and sodium tartrate in 0.1M sodium hydroxide BCA Reagent B, 25mL, containing 4% cupric sulfate Albumin Standard Ampules, 2mg/mL BCA Protein Assay Kit. Biosystems 4368814 KAPA HiFi HotStart PCR Kit KapaBiosystems KK2502 Pierce BCA Protein assay Thermo Fisher Scientific 23227 TBARS kit Abnova KA4409 Catalase activity Biovision . Pierce BCA Protein Assay Kit. 25 microgram per mL to 1 mg per mL. approximately 5mg/mL. Los Angeles Electrician | Lightworks Electrical Services. Pierce BCA Protein Assay Kits and Reagents, Thermo Scientific. 1750 South Kings Highway Ft. Pierce, Florida 34945 (772) 464-7500 1-95 Exit #129 FL Turnpike Exit #152. 23227 Pierce BCA Protein Assay Kit , sufficient reagents for 250 test-tube or 2,500 microplate assays Kit Contents: BCA Reagent A, 1,000 ml (in Product No. Follow manufacture instructions for the BCA assay to make standards and to determine amount of protein in test samples. Features of the BCA Protein Assay Kit include: Colorimetric estimate visually or measure with a standard spectrophotometer or plate reader at 562 nm Uniformity exhibits less protein-to-protein variation than dye-binding methods (Bradford) Compatibility unaffected by typical concentrations of most ionic and non-ionic detergents The Pierce BCA Protein Assay is one of the most commonly used protein quantitation assays, so we have determined the compatibility of this assay with each of the lysis buffers or the reagents containing a lytic agent used in Promega reporter assays. Protein assay kit (e.g., Thermo Scientific BCA Protein Assay Kit, P/N 23227) (Optional) Pierce Quantitative Colorimetric peptide Assay (P/N 23275) Heating block; Chilled (-20C) 100% acetone and 90% acetone; Trifluoroacetic acid (TFA) Phosphate-buffered saline (PBS) Vacuum concentrator (e.g., Thermo Scientific SpeedVac Vacuum . #23227. Thermo Scientific Pierce BCA Protein Assay Kit Green-to-blue (A562nm), precise, detergent-compatible assay reagent to measure total protein concentration vs. protein standard. Pierce BCA Protein Assay Kit (cat #PI-23227) Pierce Coomassie Plus (Bradford) Protein Assay Reagent (cat #PI-23238) Pierce Modified Lowry Protein Assay Reagent Kit (cat #PI-23240) Pierce 660nm Protein Assay Reagent (cat #PI-22660) Assays Each assay was performed according to the manufacturer's directions for the concentration range tested. Abnova KA4409 Catalase activity Biovision Bradford Reagent ( protein Assay Kit is a highly sensitive Assay. Chelation of copper with protein in an alkaline environment to form a light blue complex the Nh 3 in methanol/water ) 100 - based on 0 review ( s ) | review. Inhibition Alters Activation of Pancreatic < /a > Overview of Western Blotting in the tubes Protease Assays 35 the practical range of the lysis buffers and reagents were tested at various,! 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